|
KMID : 1007520090180020565
|
|
Food Science and Biotechnology
2009 Volume.18 No. 2 p.565 ~ p.569
|
|
|
Comparison of Preparation Methods for the Quantification of Ginsenosides in Raw Korean Ginseng
|
|
Hong Hee-Do
Sim Eun-Mi
Kim Kyung-Tack
Rho Jeong-Hae
Rhee Young-Kyoung
Cho Chang-Won
|
|
|
Abstract
|
|
|
|
This study was conducted to evaluate the effects of different preparation methods on the recovery and quantificationof ginsenosides in raw Korean ginseng (Panax ginseng C.A. Meyer). Eight major ginsenosides (Rb1, Rb2, Rb3, Rc, Rd, Re, Rf,and Rg1) were analyzed by high performance liquid chromatography (HPLC), after which the recovery and repeatability of theextraction of those ginsenosides using 3 different preparation methods were compared [A. direct extraction (DE) method, hotMeOH extraction/evaporation/direct dissolution; B. solid phase extraction (SPE) method, hot MeOH extraction/evaporation/dissolution/C18 cartridge adsorption/MeOH elution; C. liquid-liquid extraction (LLE) method, hot MeOH extraction/evaporation/dissolution/n-BuOH fractionation]. Use of the DE method resulted in a significantly higher recovery of totalginsenosides than other methods and a relatively clear peak resolution. Use of the SPE and LLE methods resulted in clearerpeak resolution, but lower ginsenoside recovery than the DE method. The LLE method showed the lowest ginsenosiderecovery and repeatability among the 3 methods. Given that the DE method employed only extraction, evaporation, and adissolution step (avoiding complicate and time consuming purification), this technique may be an effective method for thepreparation and quantification of ginsenosides from raw Korean ginseng.
|
|
|
KEYWORD
|
|
|
Korean ginseng, ginsenoside analysis, extraction, purification, high performance liquid chromatography
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|